NEGATIVE STAINING
\nˈɛɡətˌɪv stˈe͡ɪnɪŋ], \nˈɛɡətˌɪv stˈeɪnɪŋ], \n_ˈɛ_ɡ_ə_t_ˌɪ_v s_t_ˈeɪ_n_ɪ_ŋ]\
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The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
By DataStellar Co., Ltd
Word of the day
Dopamine Acetyltransferase
- An enzyme that catalyzes the of groups from acetyl-CoA to arylamines. They have wide specificity for aromatic amines, particularly serotonin, and can also catalyze acetyl transfer between arylamines without CoA. EC 2.3.1.5.